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Related Concept Videos

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
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Multiplex mRNA assay using electrophoretic tags for high-throughput gene expression analysis.

Huan Tian1, Liching Cao, Yuping Tan

  • 1ACLARA BioSciences, Inc., 1288 Pear Avenue, Mountain View, CA 94043, USA. ttian@aclara.com

Nucleic Acids Research
|September 10, 2004
PubMed
Summary
This summary is machine-generated.

This study introduces eTag molecules for multiplex mRNA quantification using capillary electrophoresis. This novel technology enables sensitive, high-throughput gene expression analysis for up to 44 targets.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Accurate quantification of multiple mRNA targets is crucial for understanding gene expression.
  • Existing methods may lack sensitivity, throughput, or multiplexing capability.

Purpose of the Study:

  • To develop and validate a novel multiplexing technology for simultaneous mRNA quantification.
  • To establish a sensitive and high-throughput platform for gene expression analysis.

Main Methods:

  • Utilized eTag molecules with distinct charge-to-mass ratios for coding mRNA targets.
  • Employed capillary electrophoresis with laser-induced fluorescence detection for quantification.
  • Integrated eTag technology with the Invader mRNA assay for isothermal linear amplification.

Main Results:

  • Successfully quantified up to 44 mRNA targets simultaneously.
  • Demonstrated sensitive detection of thousands of mRNA copies.
  • Validated the assay's precision using the Z' factor.
  • Examined inflammation-responsive genes in human umbilical vein endothelial cells.

Conclusions:

  • eTag multiplex mRNA assay offers a sensitive, high-throughput platform for gene expression analysis.
  • The synergy between eTag molecules and capillary electrophoresis enhances quantification capabilities.
  • This technology provides a unique solution for complex gene expression studies.