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The glioma-amplified sequence 41 gene (GAS41) is a direct Myb target gene

Daniel Braas1, Holger Gundelach, Karl-Heinz Klempnauer

  • 1Institut für Biochemie, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm Str.2, D-48149 Münster, Germany.

Nucleic Acids Research
|September 10, 2004
PubMed

Insights

The retroviral oncogene v-Myb directly activates the GAS41 gene, identifying it as a novel v-Myb target. This discovery sheds light on v-Myb

Area of Science:

  • Oncogenesis
  • Molecular Biology
  • Gene Regulation

Background:

  • The retroviral oncogene v-myb (v-Myb) encodes a transcription factor implicated in myelomonocytic cell transformation.
  • v-Myb is believed to deregulate target gene expression, though many such genes remain unidentified.
  • The chicken glioma-amplified sequence 41 (GAS41) gene is near the known Myb-regulated lysozyme gene and possesses potential Myb-binding sites.

Purpose of the Study:

  • To investigate whether the GAS41 gene is a direct target of the v-Myb transcription factor.
  • To elucidate the mechanism of GAS41 gene regulation by v-Myb.

Main Methods:

  • Co-transfection experiments using a v-Myb/estrogen receptor fusion protein.
  • Electrophoretic mobility-shift assays (EMSA) to assess in vitro DNA binding.
  • Chromatin immunoprecipitation (ChIP) assays to confirm in vivo binding.

Main Results:

  • GAS41 gene is directly activated by v-Myb.
  • v-Myb stimulates the GAS41 promoter activity in a DNA-binding dependent manner.
  • EMSA and ChIP assays confirm direct binding of v-Myb to the GAS41 promoter both in vitro and in vivo.
  • No significant difference in GAS41 replication origin activity was observed between cells expressing and not expressing v-Myb.

Conclusions:

  • The GAS41 gene is identified as a novel direct transcriptional target of v-Myb.
  • v-Myb's role in regulating GAS41 expression is established.
  • GAS41's replication origin activity appears independent of v-Myb expression in myelomonocytic cells.

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