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Optimal fermentation conditions for enhanced glutathione production by Saccharomyces cerevisiae FF-8
Jae-Young Cha1, Jin-Chul Park, Beong-Sam Jeon
1Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
Abstract:
The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
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