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Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Biodiversity amongst cultivable polycyclic aromatic hydrocarbon-transforming bacteria isolated from an abandoned
Chiara Zocca1, Simona Di Gregorio, Filippo Visentini
1Laboratories of Microbial Biotechnology and Environmental Microbiology, Department of Science and Technology, University of Verona, Strada Le Grazie 15 - Ca' Vignal, 37134 Verona, Italy.
Abstract:
The characterisation of a microbial community of a polycyclic aromatic hydrocarbons (PAHs) contaminated site (formerly Carbochimica, Trento, Italy) was carried out. A preliminary evaluation of the heterogeneity and the metabolic activity of the microbial community were attempted by denaturing gradient gel electrophoresis (DGGE) and reverse transcriptase-denaturing gel electrophoresis (RT-DGGE). The presence of a heterogeneous and metabolically active microbial community was found. To evaluate the PAH-transforming potential of the soil bacterial community, enrichment cultures were set up. Taxonomically diverse bacteria, showing different biochemical PAH-transforming pathways were obtained. Some of the isolates showed not nah-homologous PAH-transforming genotypes.
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