Related Experiment Video
Updated: Aug 22, 2026

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
Published on: January 7, 2019
Cyanidin-3-glucoside regulates phosphorylation of endothelial nitric oxide synthase
Jin-Wen Xu1, Katsumi Ikeda, Yukio Yamori
1Frontier Health Science, School of Human Environmental Science, MUKOGAWA Women's University, Nishinomiya 663-8179, Japan. jwxu@mwu.mukogawa-u.ac.jp
Abstract:
Dietary anthocyanins are widely distributed in fruits, vegetables and red wines, and there are several reports mentioning their beneficial effects. Previously we reported that cyanidin-3-glucoside (Cy3G) induced endothelial nitric oxide synthase (eNOS) expression and nitric oxide (NO) production in bovine vascular endothelial cells. In the present study, we show that Cy3G regulates phosphorylation of eNOS and Akt, affects the interaction between eNOS and soluble guanylyl cyclase, and increases cyclic guanosine monophosphate (cGMP) production. Our results suggest that Cy3G enhances vascular eNOS activity, and may help to improve vascular endothelial function.
More Related Videos
08:32Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
07:15Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
Published on: July 3, 2025
Related Concept Videos
Nitric Oxide Signaling Pathway
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:
Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
GPCRs Regulate Adenylyl Cylase Activity
Two...
Regulation of Angiogenesis and Blood Supply
cAMP-dependent Protein Kinase Pathways