Related Experiment Video
Updated: Aug 22, 2026

Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
Ectopic expression of a cold-inducible transcription factor, CBF1/DREB1b, in transgenic rice (Oryza sativa L.)
Sang-Choon Lee1, Kyung-Won Huh, Kyungsook An
1Department of Life Science, Sogang University, Seoul 121-742, Korea.
Abstract:
The gene encoding C-repeat/dehydration-responsive element binding factor 1 (CBF1/DREB1b) of Arabidopsis was introduced into rice (Oryza sativa L.) under the control of the maize ubiquitin promoter. Its incorporation and expression in transgenic rice plants were confirmed by DNA and RNA gel-blot analyses. Cold tolerance in the transgenics was not significantly different from that of the wild-type plants, as determined by ion leakage, chlorophyll fluorescence, and survival rates. However, the cold-responsive genes lip5, lip9, and OsDhn1 were up-regulated in the transgenic plants, suggesting that the cold signal transduction pathway involving CBF1 is partially conserved in this cold-labile plant.
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Cell Specific Gene Expression
General Transcription Factors
Reporter Genes
Commonly used reporter...
Cis-regulatory Sequences
Constitutive and Regulated Gene Expression
