Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multidimensional protein profiling technology and its application to human plasma proteome.

Kiyonaga Fujii1, Tomoyo Nakano, Takeshi Kawamura

  • 1Clinical Proteome Center, Tokyo Medical University, 2-6-1, Nishi-shinjuku Shinjuku-ku, Tokyo 163-0203, Japan.

Journal of Proteome Research
|September 14, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cancer cell immunity-related protein co-expression networks are associated with early-stage solid-predominant lung adenocarcinoma.

Frontiers in oncology·2024
Same author

Dimethylglycine, a Methionine Metabolite, Participates in the Suppressive Effect of Methionine on 1-Fluoro-2,4-dinitrobenzene-Induced Dermatitis.

Biological & pharmaceutical bulletin·2023
Same author

Perfluorooctanoic acid (PFOA) as a stimulator of estrogen receptor-negative breast cancer MDA-MB-231 cell aggressiveness: Evidence for involvement of fatty acid 2-hydroxylase (FA2H) in the stimulated cell migration.

The Journal of toxicological sciences·2022
Same author

Minichromosome maintenance 2 is an independent predictor of survival in patients with lung adenocarcinoma.

Molecular and clinical oncology·2021
Same author

Discovery of a Highly Potent and Selective Degrader Targeting Hematopoietic Prostaglandin D Synthase via In Silico Design.

Journal of medicinal chemistry·2021
Same author

Protein co-expression network-based profiles revealed from laser-microdissected cancerous cells of lung squamous-cell carcinomas.

Scientific reports·2021

A new two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC/MS/MS) system enhances proteome analysis for clinical research. This high-throughput method significantly improves protein identification and sequence coverage in complex samples like human plasma.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Clinical proteomics requires robust systems for analyzing numerous human samples.
  • Existing multidimensional liquid chromatography/tandem mass spectrometry (LC/MS/MS) systems have limitations for high-throughput clinical applications.

Purpose of the Study:

  • To develop a technically simple, high-throughput proteome analysis system for clinical proteomics.
  • To improve protein identification and sequence coverage compared to one-dimensional methods.

Main Methods:

  • Construction of a two-dimensional (2D)-LC/MS/MS system integrating strong cation exchange (SCX) chromatography and micro-reversed-phase LC/MS/MS.
  • Optimization of SCX as an off-line peptide fractionation method.
  • Evaluation using protein digest mixtures and application to human plasma proteome analysis.

Related Experiment Videos

Main Results:

  • The 2D-LC/MS/MS system more than doubled detected peptide fragments and protein sequence coverage compared to 1D-LC/MS/MS.
  • Successfully identified 174 proteins from 1 microL of human plasma (HSA and IgG depleted).
  • Achieved a total analysis run time of less than half a day.

Conclusions:

  • The developed 2D-LC/MS/MS system is a simple, high-throughput solution for clinical proteomics.
  • Demonstrates significant potential for practicing clinical proteomics efficiently.
  • Offers enhanced protein identification capabilities for complex biological samples.