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Bystander effect and adaptive response in C3H 10T(1/2) cells
S A Mitchell1, S A Marino, D J Brenner
1Center for Radiological Research, Columbia University, New York, NY 10032, USA.
International Journal of Radiation Biology
|September 14, 2004
Summary
Low doses of radiation can trigger adaptive responses that counteract the bystander effect. Radioadaptation in unirradiated cells, via transmissible factors, reduces oncogenic transformation but not cell survival.
Area of Science:
- Radiobiology
- Cellular response to radiation
Background:
- The bystander effect describes responses in non-targeted cells after irradiation.
- Adaptive response in cells can influence radiation's dose-response curve, particularly at low doses.
Purpose of the Study:
- To investigate the interplay between the bystander effect and adaptive response.
- To determine if these phenomena impact the dose-response curve at low radiation doses.
Main Methods:
- Utilized a novel apparatus for co-culturing targeted and non-targeted C3H 10T(1/2) cells.
- Investigated bystander and adaptive responses induced by supernatants from irradiated cells.
- Assessed cell killing, oncogenic transformation, and clonogenic survival.
Main Results:
- Co-culturing with alpha-particle irradiated cells induced bystander effects (cell killing, transformation).
- Low-dose X-ray pre-treatment (adaptive dose) significantly reduced the bystander effect (95% cancellation).
- Transmissible factors in supernatant from irradiated cells induced bystander effects and reduced oncogenic transformation.
Conclusions:
- Adaptive radiation doses can largely abolish the bystander effect induced by alpha-particles.
- Radioadaptation in unirradiated cells, mediated by transmissible factors, can reduce oncogenic transformation frequency.
- This radioadaptation does not appear to affect cell survival.
Keywords:
Non-programmatic