Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biomacromolecules electrostatic self-assembly on 3-dimensional tissue engineering scaffold.

Huiguang Zhu1, Jian Ji, Jiacong Shen

  • 1Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027 China.

Biomacromolecules
|September 14, 2004
PubMed
Summary

This study demonstrates that coating poly(D,L-lactide) scaffolds with poly(ethylenimine) and gelatin enhances chondrocyte growth. This polyelectrolyte multilayer technique offers a stable and effective method for modifying biomedical devices for tissue engineering and drug delivery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hepatic Porphyria Presenting with Persistent Abdominal Pain: A Case Report and Literature Review.

Iranian journal of pathology·2026
Same author

Targeted cultivation of diatoms in mariculture wastewater by nutrient regulation and UV-C irradiation.

Frontiers in microbiology·2024
Same author

Retraction notice to "Seleno-cyclodextrin sensitizes human breast cancer cells to TRAIL-induced apoptosis through DR5 induction and NF-κB suppression" [Eur J Cancer., 47 (2011) 1890-1907, http://dx.doi.org/10.1016/j.ejca.2011.04.004].

European journal of cancer (Oxford, England : 1990)·2023
Same author

Long noncoding RNA and mRNA profiling of hypothalamic-pituitary-mammary gland axis in lactating sows under heat stress.

Genomics·2020
Same author

Polymyxin B-Polysaccharide Polyion Nanocomplex with Improved Biocompatibility and Unaffected Antibacterial Activity for Acute Lung Infection Management.

Advanced healthcare materials·2020
Same author

Efficient activation of Pd/CeO<sub>2</sub> catalyst by non-thermal plasma for complete oxidation of indoor formaldehyde at room temperature.

Chemosphere·2020

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Surface Chemistry

Background:

  • Poly(D,L-lactide) (PDL-LA) scaffolds are widely used in tissue engineering.
  • Surface modification is crucial for improving scaffold biocompatibility and cell interaction.
  • Existing coating methods can be complex and lack stability.

Purpose of the Study:

  • To develop a stable, positively charged surface on PDL-LA scaffolds using polyelectrolyte multilayers.
  • To investigate the effect of poly(ethylenimine) (PEI) and gelatin coatings on chondrocyte behavior.
  • To evaluate the potential of this technique for biomedical device applications.

Main Methods:

  • Fabrication of PDL-LA scaffolds.
  • Layer-by-layer deposition of poly(ethylenimine) (PEI) and gelatin via electrostatic adsorption (ESA).

Related Experiment Videos

  • Characterization using zeta-potential, quartz crystal microbalance (QCM), scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM).
  • Assessment of chondrocyte viability, protein content, and morphology.

    • Main Results:

    • Successful creation of alternating PEI/gelatin layers on PDL-LA scaffolds, confirmed by zeta-potential and QCM.
    • Uniform gelatin distribution within the 3-D porous scaffold structure.
    • Enhanced chondrocyte viability, proliferation, and favorable cell morphology on modified scaffolds compared to unmodified ones.
    • The PEI/gelatin coating did not negatively impact scaffold porosity or integration.

    Conclusions:

    • Polyelectrolyte multilayer coatings using PEI and gelatin provide a stable and effective surface modification for PDL-LA scaffolds.
    • This method promotes chondrocyte growth, indicating potential for cartilage tissue engineering.
    • The technique is simple, stable, and offers broad applications in drug delivery and complex biomedical device surface modification.