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Updated: Aug 22, 2026
A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
[Effects of ulinastatin on renal ultrastructure after ischemia-reperfusion in rats]
Cong-cong Chen1, Yan Zhou, Zi-ming Liu
1Department of Anesthesiology, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China.
Objective:
To investigate the effect of ulinastatin on renal function and ultrastructure changes after renal ischemia-reperfusion in rats.
Methods:
Acute ischemic renal injury model was established (45 min of bilateral renal ischemia and reperfusion for 24 h). Thirty Male SD rats were randomly divided into 3 groups: sham operation group (control group or group C, without renal ischemia), renal ischemia-reperfusion group (ischemia-reperfusion group or group I, without ulinastatin), renal ischemia-reperfusion and ulinastatin intravenous injection group (ulinastatin group or group U). BUN level, serum creatinine values and renal ultrastructure were measured.
Results:
Serum creatinine (167 +/- 39) micromol/L and BUN concentration (21 +/- 7) mmol/L in group I were significantly higher than those in group U: serum creatinine (116 +/- 13) micromol/L and BUN concentration (14.1 +/- 2.6) mmol/L (P < 0.05). The renal ultrastructure was greatly injured in group I, meanwhile, it was obviously ameliorated in group U.
Conclusion:
Ulinastatin greatly improved renal function and provides remarkable protection on renal ultrastructure after ischemia-reperfusion of kidney in rats.
