Related Experiment Video
Updated: Aug 22, 2026

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
Published on: January 6, 2010
Oxygen permeability of amniotic membrane and actual tear oxygen tension beneath amniotic membrane patch
Tsuyoshi Yoshita1, Akira Kobayashi, Kazuhisa Sugiyama
1Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa, Ishikawa-prefecture 920-8641, Japan.
Purpose:
To investigate the oxygen permeability (Dk) and oxygen transmissibility (Dk/t) of amniotic membrane (AM), and tear oxygen tension beneath AM in rabbits.
Design:
Experimental study.
Method:
The water content of AM was measured to calculate Dk and Dk/t, and compared with those of a therapeutic soft contact lens (TSCL). The tear oxygen tension beneath AM and TSCL was also measured in four male albino rabbits.
Results:
The average water content of AM was significantly higher than that of TSCL (96.8 +/- 0.8% (mean +/- SD) vs 78%, P =.0006), giving rise to a significantly higher Dk and Dk/t in AM as compared with TSCL (142.8 +/- 4.7 vs 65.3, respectively, P =.0012, and 92.9 +/- 3.0 vs 29.7, respectively, P =.0008). The average tear oxygen tension under AM was also higher than TSCL (94.9 +/- 2.9 vs 59.1 +/- 4.9 mm Hg, P =.0006).
Conclusions:
These findings support the rationale of using AM as a superior bandage in treating persistent corneal epithelial defects or ulcers.
More Related Videos
Related Concept Videos
Assessment of Diffusion and Perfusion
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this principle...
Amperometry: Overview
Pulse Oximetry
Purpose
Average SpO2 values are greater than 95%. If the readings fall below 90%, it indicates that...
Oxygen Delivering System I: Nasal Cannula and Face Mask
Nasal Cannula
A nasal cannula is a lightweight tube split at one end into two prongs and placed in the nostrils. It is typically used to deliver low to medium levels of oxygen.
Suggested flow rate: The suggested flow rate for a nasal cannula typically ranges between 1 and 6 L/min.
Oxygen percentage setting:...

