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Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori

Jae Man Lee1, Takahiro Kusakabe, Yutaka Kawaguchi

  • 1Laboratory of Silkworm Science, Faculty of Agriculture, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|September 15, 2004
PubMed

Insights

This study decodes the gene for Bombyx mori translationally controlled tumor protein (BmTctp), revealing its widespread expression and complex promoter region. The findings provide insights into Tctp

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Translationally controlled tumor protein (Tctp/p23) is upregulated in tumors but also present in normal cells.
  • Understanding Tctp's expression regulation and physiological role is crucial.

Purpose of the Study:

  • To elucidate the molecular basis of Bombyx mori Tctp (BmTctp) expression.
  • To investigate the physiological significance of BmTctp.

Main Methods:

  • Deduction of BmTctp cDNA sequence from EST database.
  • RT-PCR analysis to determine BmTCTP mRNA expression.
  • Inverse PCR to obtain the genomic clone.
  • Sequence analysis of the 5'-flanking region.
  • Luciferase reporter assay for promoter activity.

Main Results:

  • BmTCTP mRNA is transcribed in all larval organs and consistently expressed during the cell cycle.
  • The BmTCTP gene contains two introns and three exons.
  • The 5'-flanking region possesses a TATA-less promoter with elements like GATA box, CCAAT, MEF2, E4BP4.01, and AP-1.
  • A specific region (-676 to +66) significantly enhances promoter activity.
  • The promoter contains enhancer and repressor elements.

Conclusions:

  • The BmTctp gene has a complex, TATA-less promoter structure.
  • Multiple regulatory elements control BmTctp gene expression.
  • BmTctp exhibits broad expression, suggesting diverse physiological roles.

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