Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

beta-Alanine synthase: purification and allosteric properties.

M M Matthews1, W Liao, K L Kvalnes-Krick

  • 1Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599-7260.

Archives of Biochemistry and Biophysics
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nivolumab plus gemcitabine-cisplatin for unresectable or metastatic urothelial carcinoma: health-related quality-of-life analyses from the phase III CheckMate 901 trial.

ESMO open·2026
Same author

Flooded with potential: urban drainage science as seen by early-career researchers.

Water science and technology : a journal of the International Association on Water Pollution Research·2025
Same author

Achievement of Target Gain Larger than Unity in an Inertial Fusion Experiment.

Physical review letters·2024
Same author

[Expression and significance of INSM1 and SOX11 in pancreatic neuroendocrine tumor and solid pseudopapillary neoplasm].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2023
Same author

Targeting the renin angiotensin system for respiratory diseases.

Advances in pharmacology (San Diego, Calif.)·2023
Same author

B-Cell-Activating Factor Contributes to Elevation of the Content of Regulatory B Cells in Neonatal Sepsis.

Bulletin of experimental biology and medicine·2023

Rat liver beta-Alanine synthase was purified and characterized. This enzyme shows ligand-induced polymerization and cooperativity, suggesting a regulatory role in beta-alanine metabolism.

Area of Science:

  • Biochemistry
  • Enzymology

Background:

  • Beta-alanine synthase is crucial for beta-alanine metabolism.
  • Understanding its properties is key to elucidating metabolic pathways.

Purpose of the Study:

  • To purify and characterize beta-alanine synthase from rat liver.
  • To investigate the enzyme's oligomeric state, kinetics, and regulatory mechanisms.

Main Methods:

  • Protein purification using affinity chromatography exploiting ligand-induced polymerization.
  • Enzyme kinetics assays (Km, kcat/Km, cooperativity).
  • Analysis of subunit molecular weight and native size.

Main Results:

  • Beta-alanine synthase purified >1000-fold with a hexameric native structure (subunit MW 42,000).

Related Experiment Videos

  • Enzyme polymerization is ligand-dependent (substrate/inhibitor association, product dissociation).
  • Exhibited positive cooperativity (nH = 1.9) towards N-carbamoyl-beta-alanine at a regulatory site.

    • Conclusions:

    • Purified beta-alanine synthase demonstrates complex regulatory behavior through polymerization.
    • Ligand-induced conformational changes are critical for enzyme activity and regulation.
    • Enzyme stability is maintained with glycerol and detergents, and sensitive to heat and reducing agents.