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Measurement of plasma volume in neonates
M Y Anthony1, S R Goodall, M Papouli
1University Department of Child Health, General Infirmary, Leeds.
Archives of Disease in Childhood
|January 1, 1992
Summary
A new method accurately measures plasma volume in critically ill newborns using indocyanine green dye dilution. This safe technique minimizes blood draws and disturbance, offering a reliable tool for neonatal care.
Area of Science:
- Neonatalogy
- Clinical Chemistry
- Pediatric Research
Background:
- Accurate plasma volume measurement is crucial for managing critically ill newborns.
- Existing methods are often invasive or unreliable in this vulnerable population.
Purpose of the Study:
- To adapt and validate the indocyanine green dye dilution technique for safe and reliable plasma volume measurement in sick neonates.
- To minimize blood sample volume and infant disturbance during measurements.
Main Methods:
- Adapted indocyanine green (ICG) dye dilution technique.
- Used diluted infant blood samples and pooled adult sera for standard curves.
- Performed paired measurements and post-infusion assessments in preterm and fullterm infants.
Main Results:
- Measured plasma volumes ranged from 21.4 to 106 ml/kg across 30 occasions in 18 infants.
- Close agreement between paired measurements was observed in 8 out of 12 infants, with small errors (0.2–5.2 ml).
- Plasma recovery errors ranged from -2.9% to +4.7% in well-matched measurements.
Conclusions:
- The adapted ICG dye dilution technique offers a safe and reliable method for measuring plasma volume in sick neonates.
- Potential sources of error, such as sampling timing and albumin distribution, were identified and discussed.
- This method provides a valuable tool for optimizing fluid management in critically ill infants.