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Library screening for D-amino-acid oxidase gene: application of real-time PCR
1Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan. konno@dokkyomed.ac.jp
Abstract:
Quantitative real-time PCR shows the quantity in addition to the presence of the target sequence. This property seemed very useful for library screening. Then, real-time PCR was employed to screen for lambda phages carrying D-amino-acid oxidase gene from mouse genomic library. Using stepwise dilution screening combined with real-time PCR, positive phages were isolated in a short time.
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