Related Experiment Video
Updated: Aug 22, 2026

Laboratory Simulation of an Iron(II)-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria
Published on: July 24, 2016
Characterization of an indigenous iron-oxidizing bacterium and its effectiveness in bioleaching heavy metals from
1Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, P.R. China.
Abstract:
The objective of the present study was to isolate the indigenous iron-oxidizing bacterium and compare its effectiveness in bioleaching of heavy metals from fresh anaerobically digested sludge and aged sludge which had undergone a storage period in a sludge holding tank. An acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans strain ANYL-1 was successfully isolated from the sludge collected from a wastewater treatment plant at Yuen Long district in Hong Kong. It was a Gram negative, non-motile rod shaped bacterium which used ferrous iron, elemental sulfur or thiosulfate as energy source, but did not utilize tetrathionate or glucose as energy source. The optimal temperature and pH for its growth and iron oxidation were 30-35 degrees C and pH 2.0-2.5, respectively. When it was used in the bioleaching of anaerobically digested sewage sludge, an inhibition on metal solubilization was observed in fresh sludge except for Zn whose dissolution was solely a chemical process. Compared to the 3 and 4 days required for solubilization of Cu and Cr respectively from the sludge sample collected after the sludge holding tank (Sludge SHT), 6 days were required to bioleach Cu and Cr from fresh sludge (Sludge AD). The fresh sewage sludge posed an unfavorable condition for bioleaching of heavy metals from anaerobically digested sludge as reflected by the prolonged bioleaching time. Therefore, further studies were needed to understand the inhibitory effects in the fresh anaerobically digested sludge and develop measures to remove it in order to improve the heavy metal bioleaching efficiency.
Related Concept Videos
Microbial Leaching
Microbes and Other Elemental Cycles
Microbial Nutrition
Metabolism of Chemolithotrophs
Acid Mine Drainage
Microbial Corrosion

