Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transient two-dimensional infrared spectroscopy: exploring the polarization dependence.

Jens Bredenbeck1, Jan Helbing, Peter Hamm

  • 1Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.

The Journal of Chemical Physics
|September 16, 2004
PubMed
Summary

We developed transient two-dimensional IR spectroscopy (T2D-IR) to study short-lived molecules. This technique uses UV and IR pulses to reveal detailed structural information about transient species.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How vibrational excitation shakes up the UV/VIS spectrum: A 2D-VE study.

The Journal of chemical physics·2026
Same author

Detecting Vibrational Energy Transfer into an Enzyme Active Site via a Transition State Analog.

The journal of physical chemistry letters·2026
Same author

Molecular Mechanism of the Oxidative Cleavage of Alkenes by Photoexcited Nitroarenes.

Journal of the American Chemical Society·2026
Same author

Signal propagation in LOV-based multidomain proteins: time-resolved infrared spectroscopy reveals the complete photocycle of YF1 and PAL.

Physical chemistry chemical physics : PCCP·2026
Same author

Monomer and dimer pathways of earth-abundant manganese tricarbonyl pre-catalysts for CO<sub>2</sub> reduction studied by time-resolved IR spectroscopy.

Physical chemistry chemical physics : PCCP·2026
Same author

Evolution and design shape protein dynamics in LOV domains - spanning picoseconds to days.

Journal of molecular biology·2025

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Chemical Dynamics

Background:

  • Transient species play crucial roles in chemical reactions and biological processes.
  • Characterizing the structure and dynamics of these short-lived intermediates is essential for understanding reaction mechanisms.
  • Existing spectroscopic techniques often lack the resolution or sensitivity to fully probe transient species.

Purpose of the Study:

  • To present a general expression for the polarization dependence of transient two-dimensional IR spectroscopy (T2D-IR).
  • To enhance the structural information content and signal manipulation versatility of T2D-IR experiments.
  • To identify optimal polarization conditions for maximizing structural insights.

Main Methods:

  • T2D-IR is a fifth-order nonlinear spectroscopic technique utilizing UV and IR laser pulses.

Related Experiment Videos

  • The method involves UV pump, narrowband IR pump, and broadband IR probe pulses.
  • Analysis focuses on the polarization dependence of the T2D-IR signals, involving up to three transition dipole moments.

    • Main Results:

    • A general framework for polarization dependence in T2D-IR is established.
    • The UV pulse introduces additional polarization control compared to traditional 2D-IR.
    • Optimal polarization conditions for maximum structural information extraction are discussed and formulated.
    • Demonstrated application of polarization selectivity in T2D-IR experiments on photoinduced electron transfer.

    Conclusions:

    • T2D-IR offers enhanced capabilities for structural determination of transient species.
    • Polarization control is a powerful tool for manipulating and interpreting T2D-IR signals.
    • This technique provides valuable insights into the dynamics of photo triggered processes, exemplified by metal-to-ligand charge transfer.