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Updated: Aug 16, 2026

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
Published on: July 8, 2010
[Effect of KyoT2 binding protein KBP1 on RBP-J-mediated transcriptional activity]
1Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To investigate the physical interaction between KyoT2 and KyoT2-binding protein 1 (KBP1) and the effect of KBP1 on RBP-J-mediated transcriptional activity.
Methods:
GST-pull down, co-immunoprecipitation, mammalian cell two hybrid assay and reporter gene assay were used to examine the physical and functional interactions between KyoT2 and KBP1.
Results:
KBP1 and KyoT2 could interact with each other both in-vitro and in-vivo, and overexpression of KBP1 could antagonize the suppressive effect of RING1 on RBP-J.
Conclusion:
KBP1 may indirectly modulate Notch signaling pathway by competing with RING1 for binding sites on KyoT2.
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