[Preparation and purification of the antibody against ribonuclease inhibitor]
Xiao-hong Shu1, Chuan-gang Li, Hai-tao Zhang
1Department of Pharmaceutical Chemistry, Dalian Medical University, Dalian 116023, China. schuxh@yahoo.com.cn
Aim:
To prepare and purify the antibody against ribonuclease inhibitor(RI).
Methods:
RI was extracted from human placenta and purified by affinity chromatography. Rabbits anti-RI antibody was obtained after immunization and then purified through rProtein A Sepharose Fast Flow chromatography column. The characters of the antibody was identified by SDS-PAGE, ELISA and Western blot.
Results:
An anti-RI antibody was obtained and purified. SDS-PAGE analysis showed that the purified anti-RI antibody has high purity. The results of Western blot and ELISA indicated that anti-RI antibody had high specificity and good stability.
Conclusion:
The anti-RI antibody with high titer, high specificity and good stability has been acquired, which lays the foundation for further research on RI.
