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Updated: Aug 22, 2026
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
[Construction and screening of the human phage antibody library from a patient with melanoma]
Jun Yang1, Tian-wen Gao, Gang Wang
1Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. YangJun8@fmmu.edu.cn
Aim:
To construct and screen the human phage antibody library from a patient with melanoma.
Methods:
Total RNA was extracted from the peripheral blood mononuclear cells (PBMCs) of a patient with melanoma who has survived for twenty years. The heavy chain Fd fragments and kappa light chains were amplified by RT-PCR. The amplified products were cloned into phagemid vector to construct Fab antibody library. The library was panned with two kinds of melanoma cells for 4 rounds.
Results:
Antibody library contained 1.2 x 10(8) different clones. Specific phage antibodies against one of the two melanoma cells were obtained after 4 rounds of panning.
Conclusion:
Specific human phage antibodies against melanoma cells were obtained.
