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Isolated basal keratinocytes express pemphigoid gestationis antigen
S E Kelly1, E McVittie, M M Black
1Department of Dermatology, University of Edinburgh, London.
The British Journal of Dermatology
|January 1, 1992
Summary
Researchers identified a specific antigen in basal keratinocytes, crucial for understanding pemphigoid gestationis. This antigen
Area of Science:
- Dermatology
- Immunology
- Cell Biology
Background:
- Pemphigoid gestationis is an autoimmune blistering disease.
- The specific antigens targeted in pemphigoid gestationis are not fully characterized.
- Basal keratinocytes form the deepest layer of the epidermis.
Purpose of the Study:
- To isolate and characterize basal keratinocytes from normal human skin.
- To identify the expression of pemphigoid gestationis-related antigen on these cells.
- To analyze the reactivity of patient sera with this antigen.
Main Methods:
- Isolation of basal keratinocytes via trypsinization of human skin.
- Identification of basal cells using collagen adherence and pemphigoid antigen presence.
- Indirect immunofluorescence assay to detect antigen expression and patient serum reactivity.
Main Results:
- Basal keratinocytes were successfully isolated and identified.
- Pemphigoid gestationis-related antigen was expressed by basal keratinocytes.
- Patient sera showed two distinct immunofluorescence patterns (polar or peripheral staining).
- The antigen was resistant to trypsinization.
Conclusions:
- Pemphigoid gestationis-related antigen is present on basal keratinocytes.
- The observed heterogeneity in immunofluorescence patterns may reflect varied antigen recognition among patients.
- Further research into antigen-antibody interactions is warranted.