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Related Experiment Videos

Keratinocyte stem cell assays: an evolving science.

Pritinder Kaur1, Amy Li, Richard Redvers

  • 1Epithelial Stem Cell Biology Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia. pritinder.kaur@petermac.org

The Journal of Investigative Dermatology. Symposium Proceedings
|September 17, 2004
PubMed
Summary
This summary is machine-generated.

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Identifying skin epithelial stem cells remains challenging. This review highlights distinct stem cell populations in hair follicles and interfollicular epidermis, emphasizing the need for improved assays.

Area of Science:

  • Dermatology
  • Stem Cell Biology
  • Cellular and Molecular Biology

Background:

  • The existence of skin epithelial stem cells has been inferred from in vivo cell kinetic studies for decades.
  • Recent advances in identifying cell surface markers have enabled prospective isolation of epidermal cells.
  • Gene marking studies confirm classical cell turnover patterns, but ex vivo identification of epidermal stem cells is controversial.

Purpose of the Study:

  • To review diverse experimental approaches for identifying keratinocyte stem cells and their underlying assumptions.
  • To discuss the current controversies and limitations in ex vivo identification of epidermal stem cells.
  • To highlight the need for more rigorous assays for stem cell activity.

Main Methods:

  • Review of existing literature on experimental approaches for identifying keratinocyte stem cells.

Related Experiment Videos

  • Analysis of assumptions underlying current ex vivo culture and identification methods.
  • Discussion of findings from gene marking studies and cell kinetic research.

    • Main Results:

    • Hair follicles and interfollicular epidermis possess distinct self-renewing stem cell populations.
    • These distinct stem cell populations contribute to specific epithelial regions during homeostasis.
    • Stem cell behavior is altered during wound healing.

    Conclusions:

    • Hair follicles and interfollicular epidermis harbor separate stem cell populations.
    • Current assays for stem cell activity require re-evaluation and improvement.
    • Discovery of new surface markers suggests potential for more accurate identification of epidermal stem cells.