The protozoan Tetrahymena as a bioindicator to screen bioactive substances

Tetrahymena thermophila has been used as a "swimming receptor" to study its chemotaxis in the presence of various bioactive substances from herbal plants. Chemotaxis of this ciliated protozoan is, in part, controlled by a cyclic GMP-dependent protein kinase (PKG), which can adjust ciliary beating. In this paper, the effects of Ginkgo biloba extract (GBE) and its main functional constituents, terpene lactones, flavonol glycosides and aglycones, on the chemotaxis and PKG activity of ciliates, were systematically investigated. GBE and its constituents exerted significant inhibition of chemotaxis and PKG activity in cells of T. thermophila. The minimal concentrations to completely inhibit chemotaxis of T. thermophila were 12, 25, 50, 100, 300, 400, 400, 500 microM, 2 mg/ml for isorhamnetin, kaempferol, quercetin, myricetin, isoquercitrin, quercetin-3-beta-d-galactoside, rutin, quercitrin, and GBE, respectively. The IC(50) values for PKG were 14, 17, 20, 25, 186, 78 microM, 0.157 mg/ml for isorhamnetin, kaempferol, quercetin, myricetin, isoquercitrin, rutin and GBE, respectively. The results indicate that the chemotaxis inhibition by GBE and its constituents and their effects on PKG are similar. This suggests that T. thermophila may be a potential experimental organism for screening other bioactive substances.