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Related Experiment Videos

Microfabricated system for parallel single-cell capillary electrophoresis.

Nigel R Munce1, Jianzhao Li, Peter R Herman

  • 1Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9.

Analytical Chemistry
|September 18, 2004
PubMed
Summary

This study presents a new platform for high-throughput single-cell electrophoresis. It uses optical tweezers and electromechanical lysis for precise cell analysis in parallel microchannels.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Single-cell electrophoresis in parallel microchannels can increase throughput and prevent cross-contamination.
  • Instrumentation requires precise spatial and temporal control of single-cell selection and lysis.

Purpose of the Study:

  • To develop a compact platform for single-cell capillary electrophoresis in parallel microchannels.
  • To integrate optical tweezers for cell selection and electromechanical lysis for cell content analysis.

Main Methods:

  • Calcein-labeled acute myeloid leukemia (AML) cells were selected using optical tweezers.
  • Cells were transported to one of four parallel microfluidic channels with laser-ablated lysis reservoirs.
  • Electromechanical lysis and simultaneous injection into parallel channels were performed.

Related Experiment Videos

  • Laser-induced fluorescence recorded electrophoretic separation of cellular contents.

    • Main Results:

    • Successful selection and lysis of individual AML cells.
    • Simultaneous injection of cellular contents into parallel microchannels.
    • Electrophoretic separation of labeled cellular contents achieved and recorded.

    Conclusions:

    • The developed platform enables high-throughput single-cell capillary electrophoresis.
    • The integrated system effectively controls cell selection, lysis, and separation.
    • This approach offers a promising method for analyzing individual cell contents.