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Updated: Aug 5, 2026
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
Quantitation of red cell-bound immunoglobulins and complement in lymphoma patients
M Podberezin1, A Levina, L Romanova
1Hematology/Oncology Dept., Children's Hospital Los Angeles, 4650 Sunset Blvd., #57, Los Angeles, CA 90027, USA.
Quantitative ELISA may be useful for determining the amount of red blood cell (RBC)-associated immunoglobulins (Igs) in patients with autoimmune hemolytic anemia (AIHA). In idiopathic AIHA, there is about 20 times more RBC-associated IgG and complement than in normal persons. In patients with low-grade lymphomas (particularly, B-CLL and splenic marginal zone lymphoma) autoimmune hemolysis is a component of their anemia. In high-grade malignant lymphomas (i.e, diffuse large B-cell lymphoma and peripheral T-cell lymphoma), as well as in Hodgkin's disease, autoimmune hemolysis contributes little, if any, anemia. The quantitative ELISA for RBC-associated IgG and complement is useful for following the effects of treatment in patients with immune hemolysis.
Quantitative ELISA may be useful for determining the amount of red blood cell (RBC)-associated immunoglobulins (Igs) in patients with autoimmune hemolytic anemia (AIHA). In idiopathic AIHA, there is about 20 times more RBC-associated IgG and complement than in normal persons. In patients with low-grade lymphomas (particularly, B-CLL and splenic marginal zone lymphoma) autoimmune hemolysis is a component of their anemia. In high-grade malignant lymphomas (i.e, diffuse large B-cell lymphoma and peripheral T-cell lymphoma), as well as in Hodgkin's disease, autoimmune hemolysis contributes little, if any, anemia. The quantitative ELISA for RBC-associated IgG and complement is useful for following the effects of treatment in patients with immune hemolysis.
