Age-related changes of the noradrenergic innervation of the uterine vasculature: a fluorescence histochemical study

Age-related changes in the density of the noradrenergic perivascular plexus supplying the uterine artery and its intrauterine branches were studied in rats 4, 12 and 24 months old using the glyoxylic acid fluorescence technique. Moreover, uterine noradrenaline levels were determined by means of high pressure liquid chromatography with electrochemical detection. No changes in the density of noradrenergic innervation or in uterine noradrenaline levels were noticeable in 12 month in comparison with 4 month rats. In contrast, a significant decrease in the density of the noradrenergic perivascular plexus of uterine artery and of its intrauterine branches as well as decrease in noradrenaline concentrations of uterine horns and cervix were observed in 24 month rats. The above findings indicate an age-related impairment of uterine noradrenergic innervation.