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Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
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Determination of human sperm calcium uptake mediated by progesterone may be useful for evaluating unexplained

Laura Cecilia Giojalas1, Pablo Iribarren, Rosa Molina

  • 1Center for Cellular and Molecular Biology, Faculty of Sciences, University of Cordoba, Cordoba, Argentina. lcgiojalas@com.uncor.edu

Fertility and Sterility
|September 18, 2004
PubMed

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During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...