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Updated: Aug 22, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse
Nicholas P Tucker1, Benoît D'Autréaux, David J Studholme
1John Innes Centre, Colney, Norwich, United Kingdom.
Abstract:
The Escherichia coli nitric oxide sensor NorR was shown to bind to the promoter region of the norVW transcription unit, forming at least two distinct complexes detectable by gel retardation. Three binding sites for NorR and two integration host factor binding sites were identified in the norR-norV intergenic region. The derived consensus sequence for NorR binding sites was used to search for novel members of the E. coli NorR regulon and to show that NorR binding sites are partially conserved in other members of the proteobacteria.
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