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Related Experiment Videos

Improved methods for controlled rapid cooling of cell suspensions.

G J Morris1, H E Richens

  • 1Asymptote Ltd., St Johns Innovation Centre, Cowley Road, Cambridge CB4 0WS, UK. jmorris@asymptote.co.uk

Cryo Letters
|September 18, 2004
PubMed
Summary

Researchers developed simple methods for rapid cooling of straws and cryovials between 100-1000°C/min. These techniques utilize direct contact with cold materials and adsorbed liquid nitrogen for efficient cryopreservation.

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Area of Science:

  • Cryobiology
  • Materials Science
  • Biotechnology

Background:

  • Achieving optimal cryopreservation requires precise control over cooling rates.
  • Traditional cooling methods may not consistently reach the rapid rates necessary for certain biological samples.

Purpose of the Study:

  • To describe simple and reproducible methods for achieving rapid cooling rates (100-1000°C/min).
  • To investigate the use of adsorbed liquid nitrogen and other materials as heat sinks for rapid cooling.

Main Methods:

  • Utilized direct contact between straws/cryovials and pre-cooled granules or plates.
  • Employed materials like activated charcoal, zeolites, and molecular sieves to adsorb liquid nitrogen.
  • Investigated controllable cooling rates using adsorbents and non-adsorbents.

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Main Results:

  • Demonstrated reproducible methods for rapid cooling in the specified temperature range.
  • Showcased the effectiveness of adsorbed liquid nitrogen in achieving high cooling rates.
  • Identified factors influencing cooling rate, including adsorbent material, granule size, and temperature.

Conclusions:

  • Simple, direct-contact methods enable rapid cooling of cryovials and straws.
  • Adsorption of liquid nitrogen into materials like activated charcoal offers a viable strategy for controlled rapid cooling.
  • Cooling rates can be precisely modulated by manipulating adsorbent properties and conditions.