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Related Experiment Videos

Extracellular recordings from patterned neuronal networks using planar microelectrode arrays.

Conrad D James1, Andrew J H Spence, Natalie M Dowell-Mesfin

  • 1Cornell University, Ithaca, NY 14850 USA. cdjame@sandia.gov

IEEE Transactions on Bio-Medical Engineering
|September 21, 2004
PubMed
Summary

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Researchers guided neuronal network formation on microelectrode arrays using patterned poly-L-lysine. This enabled the study of network activity and extracellular recordings from guided neurites.

Area of Science:

  • Neuroscience
  • Biomaterials Science
  • Bioengineering

Background:

  • Neuronal cell networks are crucial for brain function.
  • Precise control over neuronal network architecture is essential for studying neural activity.
  • Microelectrode arrays (MEAs) offer a platform for recording neuronal signals.

Purpose of the Study:

  • To develop a method for reconstructing functional neuronal cell networks on MEAs.
  • To investigate the role of patterned substrates in guiding neuronal organization and activity.
  • To achieve controlled neurite outgrowth and network formation for electrophysiological studies.

Main Methods:

  • Utilized microcontact printing (microCP) and photoresist-liftoff to pattern poly-L-lysine (PLL) on MEAs.
  • Employed haptotaxis to guide dissociated hippocampal pyramidal neurons towards localized PLL.

Related Experiment Videos

  • Designed various PLL grid patterns to control cell body attachment and neurite outgrowth.

    • Main Results:

    • Successfully reconstructed neuronal networks on MEAs with guided organization.
    • Observed bursting neuronal activity with spike amplitude attenuation.
    • Detected coincident firing activity through multichannel recordings.
    • Achieved extracellular recordings from a bundle of guided neurites.

    Conclusions:

    • Patterned PLL substrates effectively guide neuronal network formation on MEAs.
    • The reconstructed networks exhibit functional electrical activity.
    • This approach provides a platform for advanced studies in neural engineering and neuroscience.
    • Extracellular recordings from guided neurites demonstrate the potential for high-resolution neural interfacing.