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Isolation, purification, and crystallization of aspartate aminotransferase from wheat grain
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia. belj@eimb.ru
Abstract:
A procedure for isolation and purification of aspartate aminotransferase from wheat grain includes chromatography on DEAE cellulose, acidification-alkalization, precipitation with protamine sulfate, fractionation with ammonium sulfate, and chromatography on hydroxyapatite. The yield of protein was 27% with 95% purity. Crystals of the enzyme (0.05 x 0.025 x 0.015 mm3) were obtained from ammonium sulfate solution.
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