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Related Experiment Videos

High-throughput microarray detection of olfactory receptor gene expression in the mouse.

Xinmin Zhang1, Matthew Rogers, Huikai Tian

  • 1Department of Biological Sciences, Columbia University, New York, NY 10027, USA.

Proceedings of the National Academy of Sciences of the United States of America
|September 21, 2004
PubMed
Summary

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Researchers developed a high-density array to analyze olfactory receptor (OR) and vomeronasal receptor (V1R) gene expression. This tool identified specific expression patterns, revealing a correlation between genomic location and spatial expression for OR genes.

Area of Science:

  • Genomics
  • Neuroscience
  • Molecular Biology

Background:

  • Olfactory receptor (OR) and vomeronasal type 1 receptor (V1R) gene families are vast, requiring efficient methods for expression analysis.
  • Understanding the expression patterns of these receptors is crucial for deciphering olfactory and vomeronasal sensory functions.

Purpose of the Study:

  • To design and validate a high-density oligonucleotide array for high-throughput analysis of mouse OR and V1R gene expression.
  • To identify spatially and temporally regulated OR gene expression within the olfactory sensory epithelium.
  • To investigate potential correlations between the genomic organization and expression patterns of OR genes.

Main Methods:

  • Design and fabrication of a custom high-density oligonucleotide array encompassing all known mouse OR and V1R genes.

Related Experiment Videos

  • Application of the array to analyze gene expression in mouse olfactory sensory epithelium.
  • Bioinformatic analysis to assess gene expression levels, spatial distribution, and chromosomal localization.

    • Main Results:

    • The custom array successfully detected expression for a large number of olfactory receptor genes.
    • Approximately 800 OR genes, previously identified only by genomic sequence, showed specific expression in the olfactory sensory epithelium.
    • A significant correlation was observed between the spatial segregation of OR gene expression and their chromosomal location.

    Conclusions:

    • The developed high-density array is an effective tool for high-throughput analysis of olfactory and vomeronasal receptor gene expression.
    • The findings reveal novel insights into the regulation of the large OR gene family, suggesting a link between genomic structure and spatial expression patterns.
    • This study provides a foundation for further research into the functional genomics of the olfactory system.