Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development and initial characterization of xenomitochondrial mice.

I A Trounce1, M McKenzie, C A Cassar

  • 1Genomic Disorders Research Centre, and Centre for Neuroscience, University of Melbourne, Victoria, Australia.

Journal of Bioenergetics and Biomembranes
|September 21, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of national and international veterinary laboratories.

Revue scientifique et technique (International Office of Epizootics)·2021
Same author

Targeting retinal ganglion cell recovery.

Eye (London, England)·2017
Same author

Emerging Mitochondrial Therapeutic Targets in Optic Neuropathies.

Pharmacology & therapeutics·2016
Same author

Mitochondrial DNA sequence and phylogenetic evaluation of geographically disparate Sus scrofa breeds.

Animal biotechnology·2014
Same author

Xenomitochondrial mice: investigation into mitochondrial compensatory mechanisms.

Mitochondrion·2010
Same author

Effects of socialization in the household on youth susceptibility to smoking: a secondary analysis of the 2004/05 Canadian Youth Smoking Survey.

Chronic diseases in Canada·2010

Researchers created xenomitochondrial mice with diverse species mitochondria to study mitochondrial-nuclear interactions. These mice may accelerate neurodegeneration research by modeling oxidative stress and impaired energy production.

Area of Science:

  • Mitochondrial biology
  • Genetics
  • Neuroscience

Background:

  • Mitochondrial DNA (mtDNA) plays a crucial role in cellular respiration.
  • Mismatches between nuclear and mitochondrial genomes can lead to cellular dysfunction.
  • Understanding these interactions is key for studying metabolic and neurodegenerative diseases.

Purpose of the Study:

  • To generate xenomitochondrial mice with varying degrees of mitochondrial-nuclear genetic divergence.
  • To investigate the impact of mitochondrial-nuclear mismatch on cellular respiration and potential disease modeling.
  • To establish a novel mouse model for studying age-related neuronal loss and oxidative stress.

Main Methods:

  • Created xenomitochondrial mouse embryonic stem (ES) cell cybrids by fusing cytoplasts from different Mus species with mouse ES cells.

Related Experiment Videos

  • Injected these cybrids into Mus musculus domesticus (MD) ova to produce chimeric mice.
  • Assessed fertility and efficiency of producing homoplasmic offspring with introduced mitochondrial backgrounds.

    • Main Results:

    • Successfully generated chimeric mice with homoplasmic and heteroplasmic xenomitochondria from Mus spretus, Mus caroli, Mus dunni, and Mus pahari.
    • Chimeric females demonstrated high fertility rates.
    • Production of homoplasmic offspring was inefficient, with limited viable offspring carrying the introduced mitochondrial genomes.

    Conclusions:

    • Xenomitochondrial mice can be generated, but efficient production of homoplasmic offspring remains a challenge.
    • These mice represent a valuable tool for studying the consequences of mitochondrial-nuclear genetic mismatch.
    • The model holds promise for investigating accelerated age-related neuronal loss and oxidative stress in neurodegenerative disease research.