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Quantitation of Dam methyltransferase in Escherichia coli
E Boye1, M G Marinus, A Løbner-Olesen
1Department of Biophysics, Institute for Cancer Research, Oslo, Norway.
Journal of Bacteriology
|March 1, 1992
Abstract:
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.