Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DARTs: A DNA-based in vitro polypeptide display technology.

Paul de Figueiredo1, Radclyffe L Roberts, Eugene W Nester

  • 1Department of Microbiology, University of Washington, Seattle, WA 98195-7242, USA.

Proteomics
|September 21, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Single Intravenous Dose of Indole-producing Attenuated Brucella in NOD Mice: Assessment of Type 1 Diabetes Onset and Islet Immune Remodeling.

Journal of visualized experiments : JoVE·2026
Same author

Regulatory B Cells at the Crossroads of Epigenetic Control and Immune Homeostasis.

Clinical reviews in allergy & immunology·2026
Same author

PRISM: A unified platform for phage isolation and characterization from single-droplet microenvironments.

Science advances·2026
Same author

Layer-dependent modulation of <i>S. aureus</i> biofilms by light-induced oxidative stress.

Current research in microbial sciences·2026
Same author

Redefining multiple sclerosis therapy through microbial immunomodulation and epigenetic control.

Journal of translational autoimmunity·2025
Same author

CYP1B1-AS1 regulates CYP1B1 to promote Coxiella burnetii pathogenesis by inhibiting ROS and host cell death.

Nature communications·2025

We developed DNA/protein attachment and recovery tools (DARTs), a novel polypeptide display technology. DARTs enable directed evolution and protein array generation by creating robust DNA-protein hybrids for selection and purification.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Protein Engineering

Background:

  • Display technologies are crucial for linking genes to proteins, enabling directed evolution for protein property selection.
  • Existing viral and cell-based display systems have inherent synthesis and selection biases.

Purpose of the Study:

  • To introduce DNA/protein attachment and recovery tools (DARTs), a novel polypeptide display technology.
  • To overcome limitations of existing display systems by creating robust DNA-protein hybrids.

Main Methods:

  • Utilized the Agrobacterium tumefaciens protein VirD2 to generate DNA-protein hybrid molecules.
  • Constructed a truncated VirD2 variant that retained DNA linking activity without self-association.
  • Demonstrated peptide display, antibody binding for immunodetection/immunopurification, and DNA hybridization capabilities.

Related Experiment Videos

  • Purified DNA-protein hybrids via displayed peptide epitopes and amplified DNA components using PCR.

    • Main Results:

    • Developed small, robust DNA-protein hybrids using DARTs, minimizing display system biases.
    • Successfully displayed various peptides capable of antibody binding for detection and purification.
    • Showcased the ability of DNA components within hybrids to hybridize with complementary DNA.
    • Engineered a functional truncated VirD2 for efficient peptide display.

    Conclusions:

    • The DART system offers a valuable alternative to existing display technologies.
    • DARTs are suitable for directed evolution and the creation of protein arrays.
    • The technology facilitates robust DNA-protein linkage and selection processes.