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Updated: Aug 22, 2026
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
Published on: August 17, 2022
[Immunity of peritoneal monocytes against Plasmodium yoelii infected erythrocytes]
Ying-hong Mao1, Jie Yuan, Bao-feng Liu
1Department of Etiology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
To test the immunity of peritoneal monocytes against Plasmodium yoelii infected red blood cells (target cells).
Methods:
Saponinized Plasmodium yoelii infected red blood cells (SPRBC, Ghost erythrocyte) were used to immunize mice i.p twice. Three weeks later, the infected red blood cells were injected i.p.; 90 min later, the total peritoneal cells were isolated and washed for scanning electromicroscopy to observe the effects of the peritoneal monocyte to the target cell.
Results:
The peritoneal cells of the immunized mice were activated after 90 min of the challenge of target cells. The size of the cell was not even and the pili on the cell surface turned to be long and densed. Cell interconnections were found among the cells. In some peritoneal monocytes, their cell plasma were scattered (omlette-like) or with the shape as "cellular bomb". The scattered or the sheeted pili and spredding cell plasma could adhere to the target cells which were perforated densely and damaged.
Conclusion:
The protective adaptive immunity exists in the peritoneal monocytes of immunized mice.
