Theoretical model for photochemical or thermally activated immobilization of macromolecules

The covalent immobilization of macromolecules on surfaces and within 3-dimensional networks is quantitatively described using a model based on Poisson statistics. This model determines the immobilized density or layer thickness as a function of molecular weight of the macromolecule or radiant exposure prior to and following the surface deposition of the macromolecule. Measurements of immobilized layer thickness provide first-order rate constants for decomposition of the surface-bound linker molecules and an estimate of the surface-bound linker density. The model predicts the relative density of immunocomplexed antibodies as a function of the irradiation time used to immobilize antigens. By providing the average number of bonds to the immobilized molecule, the model enables studies of the effect of multiple bonds on the activity of biomolecules. Experimental data by the authors and from the literature validate the model.