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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
Published on: March 21, 2017
Interferon alpha (IFN) treatment of bone marrow stroma inhibits haematopoesis
Christoph Kasper1, Anna Lübking, Dietrich W Beelen
1Department of Medicine IV (Haematology and Oncology), Martin-Luther-University, Halle-Wittenberg, Ernst-Grube-Street 40, Halle (Saale) D-06120, Germany. christoph.kasper@web.de
Abstract:
Prolonged pre-transplant IFN administration in patients with chronic myeloid leukaemia has been associated with an increased risk of fatal transplant-related complications and an inferior outcome after allogeneic bone marrow transplantation. Using a two-stage long-term bone marrow culture as an in vitro model we were able to show a negative effect of higher doses of IFN pre-treatment of bone marrow stroma on haematopoesis. This correlated with a decline in the number of monocytes-macrophages. While monocytes-macrophages itself produce cytokines, a vicious circle may lead to an ineffective function of the microenvironment resulting in ineffective haematopoieses and increased transplant-related complications.
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