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Process implementation aspects for biocatalytic hydrocarbon oxyfunctionalization.

Bruno Bühler1, Andreas Schmid

  • 1Institute of Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zurich, Hönggerberg HPT, CH-8093.

Journal of Biotechnology
|September 24, 2004
PubMed
Summary
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Enzyme engineering and biochemical process strategies enable efficient industrial biooxidation. These advances facilitate the use of oxygenases for synthesizing valuable chemicals, overcoming previous limitations in biocatalysis.

Area of Science:

  • Biocatalysis and enzyme engineering
  • Industrial organic synthesis
  • Biochemical process engineering

Background:

  • Oxidoreductases, particularly oxygenases, perform valuable hydrocarbon oxyfunctionalizations difficult via chemical synthesis.
  • Industrial applications require overcoming challenges like enzyme activity, specificity, product degradation, cofactor recycling, and mass transfer.

Purpose of the Study:

  • To review process implementation aspects for in vivo application of oxygenases.
  • To highlight strategies for overcoming critical challenges in oxygenase-based biocatalysis.
  • To showcase advancements in enzyme and bioprocess engineering for industrial biooxidation.

Main Methods:

  • Biochemical process engineering and biocatalyst engineering approaches.
  • Heterologous overexpression of oxygenase genes.

Related Experiment Videos

  • Directed evolution, metabolic engineering, and in situ product removal.

    • Main Results:

    • Significant advances in enzyme activity, specificity, and stability achieved through engineering.
    • Successful implementation of oxygenase-based whole-cell catalysis for synthesizing chiral compounds and other valuable chemicals.
    • Demonstrated feasibility of overcoming substrate toxicity and mass transfer limitations.

    Conclusions:

    • Combined strategies in biocatalyst and bioprocess engineering facilitate efficient oxygenase-based industrial synthesis.
    • Further understanding of cell metabolism and continued engineering advancements will drive broader adoption of biooxidation processes.
    • Oxygenases offer a versatile platform for sustainable and selective chemical production.