Related Experiment Video
Updated: Aug 22, 2026

Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine
Published on: February 26, 2019
Phloridzin improves absorption of genistin in isolated rat small intestine
Wilfried Andlauer1, Jochen Kolb, Peter Fürst
1Haute Ecole Valaisanne, Route du Rawyl 47, CH-1950 Sion 2, Switzerland. anw@hevs.ch
Background & Aims:
Cancer-protective effects of isoflavones like genistin or genistein are well known. High intakes and an adequate absorption rate of isoflavones are necessary for efficient chemoprevention, though other dietary agents might increase absorption efficacy. The aim of this study was to investigate the effect of phloridzin, an inhibitor of the sodium-dependent glucose transporter (SGLT1), on genistin absorption and metabolism.
Methods:
Phloridzin and genistin were luminally administered in an isolated preparation of luminally and vascularly perfused rat small intestine. A synthetic perfusate free from blood components was used as vascular medium, with a perfluorocarbon as oxygen carrier. Luminal media consisted of a bicarbonate buffered sodium chloride solution spiked with genistin (24.5 micromol/l) and phloridzin (1 mmol/l).
Results:
In previous experiments, genistin absorption rate of 17.2% has been observed. In the present study, phloridzin administered simultaneously with genistin, increased genistin uptake 2.5 fold (44.5%).
Conclusion:
The naturally occurring substance phloridzin, present in apples, thus considerably amplify genistin absorption. These effects might offer a promising novel method in designing functional foods for cancer prevention by combining genistin- and phloridzin-containing foods.
Related Concept Videos
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Glucose Absorption Into the Small Intestine
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Drug Absorption: Factors Affecting GI Absorption
In...
Dipeptidyl Peptidase 4 Inhibitors
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
