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Updated: Aug 22, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Isoamyl alcohol-induced morphological change in Saccharomyces cerevisiae involves increases in mitochondria and cell
Kerstin Kern1, Christopher D Nunn, Alena Pichová
1Cardiff School of Biosciences, Cardiff University, P.O. Box 915, Cardiff CF10 3TL, UK.
Abstract:
Isoamyl alcohol reduced growth and induced filament formation in Saccharomyces cerevisiae. Isoamyl alcohol-induced filamentation was accompanied by an almost threefold greater increase in the specific activity of succinate dehydrogenase than in untreated cells, which suggested that isoamyl alcohol treatment caused the cells to produce more mitochondria than in normal yeast form proliferation. This was supported by measuring the dry weight of purified, isolated mitochondria. Filaments have an increased chitin content which is distributed over the majority of their surface, and is not confined to bud scars and the chitin ring between mother and daughter cells as in yeast-form cells.
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