Related Experiment Video
Updated: Aug 5, 2026
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
Published on: July 30, 2014
A novel format for a rapid sandwich EIA and its application to the identification of snake venoms
J C Cox1, A V Moisidis, J M Shepherd
1Immunodiagnostics Section, Commonwealth Serum Laboratories Limited, Parkville, Victoria, Australia.
Abstract:
A rapid sandwich enzyme immunoassay format is described where conjugate is lyophilised within the well in which the test reaction will occur. The format is straight forward to manufacture, has a prolonged shelf life, and eliminates one incubation and wash step from the usual test procedure. The technology has been applied to the development of a rapid assay for the identification of snake venom in clinical specimens. The resultant assay was specific and sensitive, provided rapid results and was appropriate for field use.
