Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CO Electroreduction Mechanism on Single-Atom Zn (101) Surfaces: Pathway to C2 Products.

Molecules (Basel, Switzerland)·2023
Same author

TGF-β1/SMAD3 Regulates Programmed Cell Death 5 That Suppresses Cardiac Fibrosis Post-Myocardial Infarction by Inhibiting HDAC3.

Circulation research·2023
Same author

CMTM3 deficiency induces cardiac hypertrophy by regulating MAPK/ERK signaling.

Biochemical and biophysical research communications·2023
Same author

Mineral and bone disorder after kidney transplantation: a single-center cohort study.

Renal failure·2023
Same author

Tailoring Dye Emissions within Metal-Organic Frameworks for Tunable Luminescence and Ratiometric Temperature Sensing.

ACS applied materials & interfaces·2023
Same author

DELAYED GREENING 409 encodes a dual-localized pentatricopeptide repeat protein required for chloroplast and mitochondrial development.

Plant physiology·2023

Related Experiment Video

Updated: Jun 11, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Nanoparticles comprising a mixed monolayer for specific bindings with biomolecules.

Ming Zheng1, Xueying Huang

  • 1Central Research and Development, Du Pont, Experimental Station, Wilmington, Delaware 19880, USA.

Journal of the American Chemical Society
|September 24, 2004
PubMed
Summary

This study introduces mixed monolayer protected nanoparticles for targeted biomolecule capture. These nanoparticles prevent nonspecific binding, enabling specific interactions with targets like proteins, crucial for diagnostics and drug delivery.

More Related Videos

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Related Experiment Videos

Last Updated: Jun 11, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Area of Science:

  • Nanotechnology
  • Biomolecular Engineering
  • Surface Chemistry

Background:

  • Nonspecific binding of biomolecules to nanoparticles hinders targeted applications.
  • Developing nanoparticles with controlled surface chemistry is essential for specific molecular recognition.

Purpose of the Study:

  • To design and synthesize mixed monolayer protected nanoparticles for specific biomolecule capture.
  • To demonstrate the efficacy of these nanoparticles in preventing nonspecific binding and achieving targeted interactions.

Main Methods:

  • Synthesis of gold nanoparticles with mixed monolayers of ethylene glycol oligomers (shielding) and specific ligands (capture).
  • Characterization using 1H NMR spectroscopy and gel electrophoresis to determine surface composition and binding properties.
  • Demonstration of specific binding to streptavidin and glutathione S-transferase (GST).

Main Results:

  • A critical ratio of shielding to capture components was identified to prevent nonspecific binding.
  • Gold nanoparticles functionalized with biotin specifically captured streptavidin.
  • Gold nanoparticles functionalized with glutathione specifically captured GST, with negligible nonspecific binding.

Conclusions:

  • Mixed monolayer protected nanoparticles offer a robust strategy for specific biomolecule recognition.
  • The shielding component effectively minimizes unwanted interactions, enhancing target specificity.
  • This approach is versatile and applicable to various target molecules in biological systems.