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Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
Published on: March 19, 2016
In vitro testing of fresh and lyophilized reconstituted human and baboon platelets
C R Valeri1, H Macgregor, M R Barnard
1Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA. navblood@nbrl.org
Transfusion
|September 24, 2004
Summary
Lyophilized platelets show altered membrane function, impairing aggregation and thromboxane production. Further in vivo studies are needed to confirm their survival and function.
Area of Science:
- Hematology
- Biotechnology
- Platelet Biology
Background:
- Platelets (PLTs) are crucial for hemostasis.
- Various preservation methods exist, including liquid, cryopreservation, and lyophilization.
- Lyophilization offers potential for long-term platelet storage.
Purpose of the Study:
- To evaluate key platelet functions and surface markers in fresh versus lyophilized reconstituted human and baboon platelets.
- To compare aggregation response, thromboxane A2 production, and surface receptor expression.
Main Methods:
- Assessed platelet aggregation and thromboxane A2 production post-stimulation.
- Measured surface markers: P-selectin, glycoprotein (GP) Ib, GPIIb-IIIa, and factor (F) V before and after stimulation.
- Compared fresh and lyophilized reconstituted platelets from humans and baboons.
Main Results:
- Fresh platelets aggregated and produced thromboxane A2 upon stimulation; surface markers P-selectin and GPIIb-IIIa increased, while GPIb decreased.
- Lyophilized reconstituted platelets showed no aggregation or thromboxane A2 production.
- Lyophilized platelets exhibited significantly greater Factor V accumulation after recalcification compared to fresh platelets.
Conclusions:
- Lyophilization alters platelet membrane function, inhibiting aggregation and thromboxane production.
- Lyophilized platelets showed impaired responses in P-selectin, GPIIb-IIIa, and GPIb expression post-stimulation.
- In vitro findings suggest potential for reduced in vivo survival of lyophilized platelets, necessitating further investigation.
