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Updated: Aug 22, 2026

Methodology for Sputum Induction and Laboratory Processing
Published on: December 17, 2017
[Sputum analysis in patients with "disharmony of stomach"]
1Department of Internal Medicine, Second Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510095, China.
Objective:
To analyze the amount and inflammatory components of the sputum in patients with "disharmony of stomach", including gastroesophageal reflux disease, peptic ulcer and chronic gastritis.
Methods:
Sputum production was induced by inspiration of aerosol of 3.5% hypertonic saline with ultrasonic nebulizer, and the total cell count in the sputum was examined. Sputum smear stained with Gimsa was used in differential counting under light microscope. Bacterial culture in the sputum was performed in the usual way. Peak expiratory flow (PEF) was tested to study the airway responsiveness.
Results:
The amount of sputum, the total cells and eosinophils in the sputum of the patients with "disharmony of stomach" were higher than those of the control. PEF decreased following sputum induction. These parameters returned to control level after treatment with Chinese herbs. Bacterial culture was negative.
Conclusion:
"Disharmony of stomach" can cause the bronchial inflammation (non-infection) characterized by eosinophil increase.
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