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Updated: Aug 22, 2026

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Published on: July 6, 2012
Efficient stimulation of site-specific ribosome frameshifting by antisense oligonucleotides
Michael T Howard1, Raymond F Gesteland, John F Atkins
1Department of Human Genetics, University of Utah, 15 N. 2030 E., Rm. 7410, Salt Lake City, UT 84112-5330, USA. mhoward@genetics.utah.edu
Abstract:
Evidence is presented that morpholino, 2'-O-methyl, phosphorothioate, and RNA antisense oligonucleotides can direct site-specific -1 translational frameshifting when annealed to mRNA downstream from sequences where the P- and A-site tRNAs are both capable of repairing with -1 frame codons. The efficiency of ribosomes shifting into the new frame can be as high as 40%, determined by the sequence of the frameshift site, as well as the location, sequence composition, and modification of the antisense oligonucleotide. These results demonstrate that a perfect duplex formed by complementary oligonucleotides is sufficient to induce high level -1 frameshifting. The implications for the mechanism of action of natural programmed translational frameshift stimulators are discussed.
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