Related Experiment Video
Updated: Aug 22, 2026
The Mouse Isolated Perfused Kidney Technique
Published on: November 17, 2016
[Hydrodynamic and biochemical adaptation to perfusion pressure profile in isolated hypothermic renal perfusion]
E Lledó García1, C Hernández Fernández, J I Martínez-Salamanca
1Servicio de Urología, Hospital General Universitario Gregorio Marañón, Madrid.
Objectives:
To evaluate hydrodynamic, biochemical and pathologic consequences of positive forced pressure on hypothermic isolated renal perfusions in comparison to softly progressive administration of perfusion solution.
Material And Method:
16 hypothermic experimental pig renal perfusion o 180 min completed under constant flow conditions. A vacuum computer-controlled pump was used. A comparison was performed of the results obtained by two groups of organs (forced perfusion pressure -I.B.- and soft perfusion pressure -I.S.).
Results:
Even though perfusion flor did not offer significant differences among both groups at the end of the procedure, I.B. group showed medium perfusion pressure and renal vascular resistance values significantly higher. Nitric oxide metabolites concentration was also greater in I.B. group. Finally, these kidneys showed more tubular cell aedema and glomerular shrinkage in comparison to I.S. group.
Conclusions:
Sharp and forced hypothermic perfusion of the isolated kidney don't increase flow perfusion values. Inversely, hydrodynamic and pathologic results are worst in comparison to soft and progressive organ perfusion.
