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Related Experiment Videos

Physiochemical interactions at the interfaces between self-etch adhesive systems and dentine.

Yong Wang1, Paulette Spencer

  • 1Department of Oral Biology, School of Dentistry, University of Missouri-Kansas City, 64108, USA. wangyo@umkc.edu

Journal of Dentistry
|September 25, 2004
PubMed
Summary

This study compared three self-etching dental adhesives, revealing differences in hybrid layer thickness and demineralization depth. Aggressive one-step systems created more complex interfaces than two-step systems.

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Area of Science:

  • Dental Materials Science
  • Biomaterials Engineering
  • Adhesion Science

Background:

  • Self-etching dental adhesives are crucial for bonding restorative materials to tooth structure.
  • Understanding the interface between adhesive and dentine is key to long-term restoration success.
  • Variations in self-etching primer/adhesive chemistry can influence interface morphology and quality.

Purpose of the Study:

  • To comprehensively analyze the morphology, quality, and chemistry of interfaces formed by three self-etching primers/adhesives with dentine.
  • To compare the characteristics of a two-step self-etching adhesive with two one-step self-etching adhesives.
  • To evaluate the influence of adhesive aggressiveness on the hybrid layer and demineralized dentine.

Main Methods:

  • Three commercial self-etching systems (Clearfil SE Bond, One-Up Bond F, Prompt L-Pop) were applied to human third molar dentine.

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  • Specimens were prepared as 5-micron-thick sections for light microscopy (Goldner's trichrome stain).
  • Micro-Raman spectroscopy and scanning electron microscopy (SEM) were used for complementary analysis.

    • Main Results:

    • Different hybrid layer thicknesses were observed, ranging from 1 to 3 micrometers depending on the system.
    • Staining revealed variations in how effectively adhesives encapsulated demineralized dentine matrix and collagen fibrils.
    • Prompt L-Pop exhibited the highest aggressiveness, significantly demineralizing subsurface dentine, while Clearfil SE Bond showed milder effects.

    Conclusions:

    • Complementary techniques (SEM, staining, micro-Raman spectroscopy) are effective for characterizing adhesive/dentine interfaces.
    • One-step self-etching systems, particularly aggressive ones, result in more complex interfaces compared to two-step systems.
    • The degree of demineralization and hybrid layer formation varies significantly among different self-etching adhesive systems.