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Reduced-bore monolithic silica column modified with C8-TEOS for reversed-phase electrochromatography
Qishu Qu1, Rongjun Qu, Qiang Xu
1Department of Chemistry, Yantai Normal University, Yantai, Shandong 264025, P.R. China. qsqu7323@hotmail.com
Journal of Separation Science
|September 25, 2004
Summary
Large diameter monolithic silica columns were developed for electrochromatography. These columns enhance sample loadability and concentration detectability, enabling semi-preparative separations.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Monolithic silica columns offer advantages in chromatography.
- Capillary electrochromatography (CEC) faces challenges with sample loading and detection limits.
Purpose of the Study:
- To develop and evaluate large-diameter monolithic silica columns for electrochromatography.
- To improve sample loadability and concentration detectability in electrochromatography.
Main Methods:
- Preparation of monolithic silica columns (2.7 mm ID) using on-column sol-gel reaction with C8-TEOS and TEOS.
- Addition of fine quartz sand to reduce Joule heating during column preparation.
- Analysis of aromatic hydrocarbons using 60% acetonitrile and 40% Tris-HCl buffer.
Main Results:
- Achieved 21,000 theoretical plates for aromatic hydrocarbons.
- Improved surface area and separation reproducibility via sol-gel coating.
- Significantly reduced Joule heating.
- Demonstrated enhanced sample loadability and concentration detectability compared to traditional CEC.
Conclusions:
- Large-diameter monolithic silica columns are promising for electrochromatography.
- These columns facilitate semi-preparative separations.
- Further optimization may enhance separation efficiency to match capillary CEC.