Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk of minor congenital heart defect in infants conceived via assisted reproductive technology: cohort study from the Copenhagen Baby Heart Study.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026
Same author

Obstetric and perinatal risks in 4601 singletons and 884 twins conceived after fresh blastocyst transfers: a Nordic study from the CoNARTaS group.

Human reproduction (Oxford, England)·2020
Same author

Cumulative live birth rate prognosis based on the number of aspirated oocytes in previous ART cycles.

Human reproduction (Oxford, England)·2018
Same author

Recurrence quantification analysis of resting state EEG signals in autism spectrum disorder - a systematic methodological exploration of technical and demographic confounders in the search for biomarkers.

BMC medicine·2018
Same author

Prostate artery embolisation for benign prostatic hyperplasia: a systematic review and meta-analysis.

European radiology·2018
Same author

The long-term prognosis for live birth in couples initiating fertility treatments.

Human reproduction (Oxford, England)·2017

Related Experiment Video

Updated: Jul 11, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
13:01

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

Ham-2 corrects the class I antigen-processing defect in RMA-S cells.

M Attaya1, S Jameson, C K Martinez

  • 1Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0678.

Nature
|February 13, 1992
PubMed
Summary

The Ham-2 gene restores antigen presentation in defective cells, suggesting both Ham-1 and Ham-2 transporters are crucial for major histocompatibility complex (MHC) class I antigen processing. A Ham-1/Ham-2 heterodimer may form the functional transporter.

More Related Videos

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice
08:43

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Published on: January 31, 2020

Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells
09:12

Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells

Published on: June 14, 2024

Related Experiment Videos

Last Updated: Jul 11, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
13:01

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice
08:43

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Published on: January 31, 2020

Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells
09:12

Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells

Published on: June 14, 2024

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The murine major histocompatibility complex (MHC) includes Ham-1 and Ham-2 genes, encoding ATP-dependent transport proteins.
  • These proteins are hypothesized to transport peptides from the cytoplasm to the endoplasmic reticulum for MHC class I binding.
  • Previous studies showed human homologue PSF-1 could rescue class I expression in defective human cells.

Purpose of the Study:

  • To investigate the role of the Ham-2 gene in antigen processing.
  • To determine if Ham-2 can correct the antigen-processing defect in the RMA-S cell line.
  • To elucidate the function of MHC-linked transporter genes in antigen presentation.

Main Methods:

  • Transfection of the RMA-S mutant murine cell line with a cloned Ham-2 gene.
  • Assessing the recovery of antigen processing and presentation to cytotoxic T lymphocytes.
  • Evaluating the surface expression of MHC class I molecules.

Main Results:

  • Expression of Ham-2 in RMA-S cells restored the ability to process and present class I-restricted antigens.
  • Partial recovery of class I surface expression was observed.
  • Ham-2 corrected processing defects for both classical (H-2 K, D) and non-classical (Qa1, HMT) MHC class I molecules.

Conclusions:

  • Both MHC-linked transporter genes, Ham-1 and Ham-2, are likely essential for MHC class I antigen processing.
  • The functional transporter in this pathway may be a heterodimer composed of Ham-1 and Ham-2 proteins.